Pdf biotechnology which is synonymous with genetic engineering. The first step in rdna technology is to isolate the desired dna in its pure form i. Recombinant dna technology was first developed in the 1970s. Recombinant dna refers to the creation of new combinations of dna segments that are not found. The first step in transformation is to select a piece of dna to be inserted into a vector. What are the steps in recombinant dna technology rdna technology. Video lecture on steps in recombinant dna technology from biotechnology process and application chapter of biology class 12 for hsc, cbse. In generally, a recombinant dna technology has five steps. A rapid extraction procedure for screening recombinant plasmid dna. Recombinant dna technology dna vectors cloning vector and expression vector. A series of steps, including the use of protease enzymes to strip proteins from the dna, are. Recombinant dna technology lecture basics of recombinant. We will be discussing the basic steps involved in rdna technology, gene cloning or.
Steps in recombinant dna technology or rdna technology youtube. To use recombinant dna technology to functionally analyze mutations introduced into cloned eukaryotic genes, a rapid procedure is necessary to assay the steps along the gene expression pathway. Recombinant dna technology is playing a vital role in improving. Pdf recombinant dna technology and its applications. Recombinant dna technology the centre for plant sciences. Recombinant dna technology development and applications. This is one of the most watched video on rdna technology on youtube with your support.
667 1507 472 332 657 1492 588 1527 454 2 856 204 597 1325 1499 1525 1124 939 441 416 226 1478 1639 148 1277 1224 1030 1315 881 1478 1151 942 1283